Studies on the Lipase Production by Aspergillus Niger through Solid State Fermentation
نویسندگان
چکیده
The current study includes isolation and screening of different fungal cultures from soil samples for lipase production by solid state fermentation. Seven different lipolytic fungal strains were isolated and screened for the purpose. These isolates were identified on the basis of morphological and microscopic studies. Among these fungal strains, Aspergillus niger gave best reproducible results and hence selected for further studies. Oil substrates were optimized and maximum lipase activity of 5.12±0.059 U/ml was observed using olive oil as substrate in a medium moistened with a diluent containing (g/ml): MgSO4.7H2O, 0.05; KH2PO4, 0.1; KCl, 0.05; ; FeSO4, 0.001; glucose, 0.8 and peptone, 2.0 (pH 6.5). Maximum lipase activity was observed for an incubation period of 72 hrs at 30oC.
منابع مشابه
Lipase Production in Solid State Fermentation Using Aspergillus niger: Response Surface Methodology
Among enzymes, lipases have been widely investigated because of the numerous industrial applications. In this study, optimization of lipase production by Aspergillus niger in solid state fermentation from rice bran as solid substrate was investigated. The optimal conditions with the aid of central composite design (CCD) under response surface methodology (RSM) were obtained. In the analysis of...
متن کاملSolid State Fermentation for Production of Chitosan by Aspergillus Niger
The effect of Solid State Fermentation (SSF) on Chitosan production by A. niger was investigated. A. niger BBRC 20004 from Biochemical and Bioenvironmental Research Centre of Sharif University of Technology (Tehran, Iran), was grown on corn residue. Chitosan was extracted from the fungal mycelia using hot alkaline and acid treatment. A. niger was incubated for 12 days on corn residue with moist...
متن کاملXylanase Production under Solid State Fermentation by Aspergillus niger
Central composite orthogonal design was applied to quantify relations of xylanase production, loss of dry matter and change of pH with four critical variables during solid state fermentation of a mixture of wheat bran and wheat straw on which Aspergillus niger CCUG 33991 was cultivated. The studied variables included the percentage of wheat straw, temperature, moisture content, and fermentati...
متن کاملInfluence of Different Nitrogen Sources on Amount of Chitosan Production by Aspergillus niger in Solid State Fermentation
In this study the effect of different nitrogen source substrates on the amount of chitosan production by Aspergillus niger was investigated. A. niger PTCC 5012 from the Persian Type Culture Collection (PTCC) was grown on soy bean, corn seed and canola residues at 30 °C for specified cultivation days under sterilized conditions. ...
متن کاملIntegrated use of residues from olive mill and winery for lipase production by solid state fermentation with Aspergillus sp.
Two-phase olive mill waste (TPOMW) is presently the major waste produced by the olive mill industry. This waste has potential to be used as substrate for solid state fermentation (SSF) despite of its high concentration of phenolic compounds and low nitrogen content. In this work, it is demonstrated that mixtures of TPOMW with winery wastes support the production of lipase by Aspergillus spp. By...
متن کامل